IGF-II mRNA expression in LI human glioblastoma cell line parallels cell growth.

ANTISENSE RNA TO THE TYPE I INSULIN-LIKE GROWTH FACTOR RECEPTOR REVERSED THE TRANSFORMED PHENOTYPE OF PC-3 HUMAN PROSTATE CANCER CELL LINE IN VITRO

The insulin-like growth factor I receptor (IGF-IR) plays an essential role in the establishment and maintenance of transformed phenotype. Interference with the IGF-IR pathway by antisense causes reversal of the transformed phenotype in many rodent and human tumor cell lines. We stably transfected the PC-3 human prostate cancer cell line with an IGF-IR antisense RNA expression plasmid. The ...

The effect of Citrullus colocynthis on the induction of Casp3 and Casp8 gene expression on human Glioblastoma U87 cell line

Introduction: Using in vitro MTT assay and Real-time PCR, the cytotoxicity and cell inhibition mechanism of using Citrullus colocynthis on human glioblastoma U87 cell line was investigated. Methods: The plant extract was extracted using ethanol (70%). To investigate the cytotoxic effects of Citrullus colocynthis extract on U87 cell line, 10 4 cells per well was incubated with different concentr...

Investigation of the Impact of Foretinib, an Oral Multikinase, on AURKA and AURKB Expression in T98 Glioblastoma Cell Line

Background/Objective: Gliomas are the most common of the primary brain tumors and accounted for more than 40% of all central nervous system (CNS) tumors. Glioblastoma (GBM) remains one of the most fatal human malignancies because of its high angiogenic. Foretinib is an oral multikinase inhibitor that represented antitumor activity in clinical studies.  AURKA and AURKB genes have been shown to b...

Down-Regulation of CD14 Transcripts in Human Glioblastoma Cell Line U87 MG

Background: Pattern recognition receptors (PRRs) are the main sensors of pathogen and danger signals in innate immunity of which Toll Like Receptors (TLRs) are the most studied ones. The contribution of PRRs in cerebral inflammation induced by microbial infection, tissue damage and cancer has not extensively been addressed so far. Glioma is the most common tumor of the central nervous system an...

Gene Expression Quantification of Toll like Receptors 2, 4 and Co-molecules in Human Glioblastoma Cell Line (U87-MG): Toward a New In vitro Model of Inflammation